||Tissue underwent exhaustive tests. The parameters studied were sampling, freezing, preservation conditions, hygrometry and temperature during vaginal tissue tests, and the rate of deformation during tests. As previously noted, vaginal tissue is anisotropic and the collection has been tested previously. We noted that freezing tissue had no consequences on the mechanical response of tissue during unidirectional tension testing. The experimental conditions that we defined (temperature, hygrometry and rate of deformation) allowed us to have reproducible tests.